Line scan - structured illumination microscopy super-resolution imaging in thick fluorescent samples
نویسندگان
چکیده
منابع مشابه
Multifocus structured illumination microscopy for fast volumetric super-resolution imaging.
We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diff...
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Many biological structures of interest are beyond the diffraction limit of conventional microscopes and their visualization requires application of super-resolution techniques. Such techniques have found remarkable success in surpassing the diffraction limit to achieve sub-diffraction limited resolution; however, they are predominantly limited to fluorescent samples. Here, we introduce a non-fl...
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Aurélie Jost, Elen Tolstik, Bo-Jui Chang, Ernst H.K. Stelzer, Anne Sentenac and Rainer Heintzmann Institute of Physical Chemistry, Abbe Center of Photonics, Friedrich-SchillerUniversity Jena, Helmholtzweg 4, 07743 Jena, Germany Leibniz Institute of Photonic Technology, Albert-Einstein Str. 9, 07745 Jena, Germany BMLS, Physical Biology, Goethe University, D-60438 Frankfurt am Main, Germany Insti...
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Three-dimensional structured illumination microscopy (3D-SIM) is a versatile and accessible method for super-resolution fluorescence imaging, but generating high-quality data is challenging, particularly for non-specialist users. We present SIMcheck, a suite of ImageJ plugins enabling users to identify and avoid common problems with 3D-SIM data, and assess resolution and data quality through ob...
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Recent advances in optical microscopy have enabled biological imaging beyond the diffraction limit at nanometer resolution. A general feature of most of the techniques based on photoactivated localization microscopy (PALM) or stochastic optical reconstruction microscopy (STORM) has been the use of thin biological samples in combination with total internal reflection, thus limiting the imaging d...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2012
ISSN: 1094-4087
DOI: 10.1364/oe.20.024167